Regulatory

Part:BBa_K1051300:Design

Designed by: Kequan Lin   Group: iGEM13_Shenzhen_BGIC_ATCG   (2013-06-30)


cln2 promoter (G1 phase to S phase)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 511


Design Notes

We get the sequence of the cln2 from a yeast database (http://www.ncbi.nlm.nih.gov/CBBresearch/Landsman/Cell_cycle_data/) .You can amplify it from the yeast gene using PCR .See the primers as follows : 

upstream:GTTTCTTCGAATTCGCGGCCGCTTCTAGAGATTTGTCTCAACGGAAGTAC

downstream:GTTTCTTCCTGCAGCGGCCGCTACTAGTATGTCTGTCGTTAAATTTAAT


Source

ATTTGTCTCAACGGAAGTACACGAAATTCGTCCCGCTGAACCTCAAAACTGCGTGTTCTA GTCACGAAACGCGCCAAAAACCGTTATGTTTCCGCATGCGGATACCTAGCGAAGAGCACA TTTGGCGTAGCCTGTTAAGTATAACTGCTAAAAATTGAAATGAACACCAACTGAAACAAC TGTTGGCCCTTTTTGAGGATCTAACCTGCGAAATGTTGATCTGACAGGCCTACTGAAAAA CGGAAAGGAAAAAAGGCCTGCGCAAACGCAGAACGCAGATCCGCCTTCTATTTGCGTATC GCACGCAGCCTCTGGCTACTTGTTTAACTTACTATCTCTTTCATATATAAAAAAATAGCA ATCGACTCTGGTAACTATTATGCTCCTCTTACTGGTTTTTTAGTGAATTGGCAATAGATA ATATCAAGGACAACTTACTTTTATTTTAACATCTACTTTACTTCATCGTCAAATTTTCTT CTTATATACAGCATTGCTAGACTCTATAGCTGCCAATTCATTCGCTTACCACATCATAAT TTGCATACAAAAGAAAATCTTTTCTTTTTTCCATTCATTCATTAA ATTTA ACGACAGACA


References